![]() ![]() PH electrode (optional) CAUTION! Never leave the pH electrode in the flow cell for any period of time when the system is not used, since this might cause the glass membrane of the electrode to dry out. Repeat with a bacteriostatic solution such as 20% ethanol (not the pH electrode, see separate instructions below). Submerge the inlet tubings in distilled water. If the system will be used with other buffers next day, rinse the pump and the system with distilled water using the PumpWash instruction as follows:ġ. The system can be left filled with a buffer overnight. If the column is to be left in the flow path, make sure that the maximum allowed flow and pressure for the column are not exceeded.įor column cleaning procedures and column storage instructions, please refer to the Instructions supplied with the column. To bypass the column, use a piece of i.d. The procedures described below are for system cleaning. You may also find that you need to tighten the connectors on your system slightly to prevent leaks when you bring a system out from the cold, and loosen them slightly before you put a system into the cold to prevent pressure build up.Ĭhanges in temperature can also affect the viscosity of your buffers so keep a close eye on your back pressure. When moving a system to or from a cold room, time must be allowed for the system to adjust to its new temperature. Cold cabinets can provide an effective solution to running your ÂKTA system in the cold whilst protecting the PC. When installing an ÄKTA system in a cold room the computer can be positioned up to 15 m away, allowing it to be positioned outside the cold room. The computer systems are not however cold room compatible, and can be damaged by being placed at 4C. All our ÄKTA systems, Ettan LC, Ettan microLC, Ettan nanoLC and fraction collectors are suitable for use in the temperature range of 4-40C. ![]() Purifying some samples at room temperature can lead to increased levels of degradation performing your purifications at 4C can help. Having the lamp and filter housings incorrectly alignedĬan result in a low light intensity warning. (marked by a filled white circle) and one for all other wavelengths Positions for aligning the Hg lamp with the filter housing, one for 280 nm This error can also be caused by incorrect positioning of the lamp. If you are using an ÄKTAFPLC, ÄKTAxpress or ÄKTAprime Therefore, the first thing that you should do if you get this message is give your ‛Error 71: WARNING low light intensity’ is not lamp failure but a dirty flow cell. One of the most common causes for the error message In the cold room the average lifetime is reduced to The system is operated at room temperature with a wavelength of 254 nm, the average Wavelengths are selected using a band pass filter. MicroLC, Ettan nanoLC and the Ettan MDLC uses a Zn lamp for monitoring at 214 nm and a Hg lampĪt all other wavelengths. The UV monitoring system of the ÄKTAprime, ÄKTAxpress, ÄKTAFPLC, Ettan This extends its lifespan, meaning the average lamp can withstand many years Xenon lampsĮmit a high intensity continuous spectrum of light, your chosen wavelengths are UV-900 monitor, the light source for which is a xenon flash lamp. The ÄKTAbasic, ÄKTApurifier, ÄKTAexplorer, ÄKTAmicro and Ettan LC systems contain a I am getting the error message ‛Error 71: WARNING low light intensity’. How long should the UV LAMP on my system Last?
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